| Primary Identifier | MGI:6741072 | Allele Type | Targeted |
| Attribute String | Epitope tag, Inserted expressed sequence | Gene | Igh |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Heavy-chain enhancer Emu, downstream of the J gene segment, was replaced with a construct consisting of a 1.5 kb segment of genomic 5' flanking sequence from the mouse Myc gene with the P1/P2 promoters, the first noncoding exon, a mouse Myc intron-less cDNA clone with an in-frame 6x histidine tag sequence on the 3' end, a short segment of the 3' UTR including the major polyadenylation signal, and a loxP site flanked neomycin resistance gene cassette, all in opposite transcriptional orientation compared to the host gene. The neo cassette was removed through subsequent cre-mediated recombination. |
