| Primary Identifier | MGI:6729296 | Allele Type | Endonuclease-mediated |
| Attribute String | Modified regulatory region | Gene | Tnf |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | The adenylate-uridylate-rich element (AU-rich element; ARE) and new regulatory element (NRE) in the 3' UTR were targeted with gRNAs (targeting GTGCAAATATAAATAGAGGG, TGCTTATGAATGTATTTATT, TTGTCTTAATAACGCTGATT and ATTTCTCTCAATGACCCGTA) using CRISPR/Cas9 technology, resulting in a 71 bp and a 76 bp deletion (TCTATTTATATTTGCACTTATTATTTATTATTTATTTATTATTTATTTATTTGCTTATGAATGTATTTATT and GCTGATTTGGTGACCAGGCTGTCGCTACATCACTGAACCTCTGCTCCCCACGGGAGCCGTGACTGTAATCGCCCTA). |
