| Primary Identifier | MGI:6720298 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Card19 |
| Transmission | Germline | Strain of Origin | 129S/SvEv |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector is designed to replace exons 3-6 (encoding the C-terminal half of the CARD and the entire CARD19 C-terminus) with a betageo-puro cassette. Absence of full-length protein expression was confirmed by western blot analysis of homozygous CD8+ T cells. Subsequent RNAseq and western blotting revealed hypomorphic expression of the adjacent Ninj1 gene. |
