| Primary Identifier | MGI:6720837 | Allele Type | Targeted |
| Attribute String | Recombinase | Gene | Fezf2 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | The allele was generated by inserting a 2A-flpo expression cassette in-frame before the STOP codon of the Fezf2 gene. The targeting vectors generated using BAC clones from the RPCI-23&24 library were electroporated into a 129SVj/B6 hybrid ES cell line (v6.5). The Resulting ES cell clones were used for tetraploid complementation to obtain male heterozygous mice. The neo selection cassette was excised by crossing to cre expressing mice. |
