Primary Identifier | MGI:6810171 | Allele Type | Targeted |
Attribute String | Epitope tag, Reporter | Gene | Fyb1 |
Transmission | Germline | Strain of Origin | C57BL/6N |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Sequence for a OneâSTrEPâtag (OST), two STOP codons, an IRES2, Kozak sequence, the cyan (teal) fluorescent reporter gene mTFP1, and an FRT site flanked neomycin resistance gene cassette, was inserted in-frame at the 3â² end of the coding sequence. The neo cassette was removed through subsequent Flp-mediated recombination. |