| Primary Identifier | MGI:6762184 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rgs3 |
| Transmission | Germline | Strain of Origin | 129S1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | By homologous recombination in ES cells, a loxP site was inserted into the mouse Rgs3 gene at intron1 and another loxP followed by a phospho-glycerate kinase-1-neo-bpA cassette flanked by FRT was inserted into intron 5. The resulted allele has Rgs3 gene exon 2 through exon 5 coding for PDZ domain flanked by loxP followed by a FRT flanked neo selection cassette. Cre-mediated recombination in germ cells resulted in the deletion of exon 2 through exon 5. The loss of Rgs3 mRNA was confirmed by RTPCR and the loss of full-length protein was also confirmed with western blot. |
