| Primary Identifier | MGI:6763019 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Prkdc |
| Strain of Origin | NOD/ShiLtJ | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/cas9 genome editing is used to create a 390-nt deletion that resulted in the complete loss of exon 5 (as well as 119bp upstream and 162bp downstream). This is designed to create a knock-out allele resulting in a premature stop codon at amino acid 135. Prkdc transcript Prkdc-201 was used as reference for the exon number and the guide sequences. |
