| Primary Identifier | MGI:6724571 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Cct6b |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 technology generated a 5bp deletion (c.373_377delAACTG) in exon 4, with the glutamate at position 125 as the first amino acid changed, shifting the reading frame, replacing it for a glycine and terminating at position Ter22 (p.Glu125GlyfsTer22). Western blotting and qPCR confirmed the absence of protein and reduced levels of mRNA in the testis, respectively. |
