| Primary Identifier | MGI:6752560 | Allele Type | Targeted |
| Attribute String | Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A vector, which contains (5' to 3') a splice acceptor, floxed neo/stop cassette and sequence encoding mutated nuclear localized lacZ with intragenic duplication corresponding to nucleotides 870-1987 after the ATG such that a shift in codon phase introduces a TGA STOP codon at nucleotide 1993 was inserted into the locus via homologous recombination. The parental Gt(ROSA)26Sortm1Etz was crossed to cre deleter to remove the floxed neo/stop cassette. The duplication in the lacZ sequence truncated b-galactosidase molecule making the lacZ inactive, except in small fraction of cells undergone infrequent spontaneous random intragenic homologous recombination restoring the functional lacZ sequence. |
