| Primary Identifier | MGI:6836755 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Prrx1-Shh*)NPtch |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A mouse sonic hedgehog cDNA lacking the sequence for the cholesterol attachment and with a p.C25S mutation (to prevent palmitoylation of the peptide) was placed under the control of the mouse paired related homeobox 1 enhancer to drive its expression in embryonic limb buds. The NonâCholesterolâ© Modified mutation involves inserting a loxP site flanked neomycin resistance gene cassette into the coding region in exon 3 upstream of the cholesterylation site, which results in a premature stop codon after glycine codon 198. The neo cassette was removed through subsequent cre-mediated recombination. |
