| Primary Identifier | MGI:6780026 | Allele Type | Endonuclease-mediated |
| Gene | Edil3 | Strain of Origin | C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Aspartic acid codon 98 (GAC) was targeted for mutation to a glutamic acid codon (GAG)(p.D98E) with an sgRNA (UAUCGAGGAGACACAUUCAUGUUUUAGAGCUAGAAAUAGCAAGUUAAAAUAAGGCUAGUCCGUUAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUUU), an ssODN template (TTTCAGGTCCCTGCATCCCTAACCCATGCCATAACGGAGGAACCTGTGAGATAAGCGAAGCCTATCGAGGAGAGACGTTTATAGGCTATGTTTGTAAATGTCCTCGGGGATTTAATGGGATTCACTGTCAGCACAGTAAGTTATTT) and a crRNA (targeting TATCGAGGAGACACATTCA) using CRISPR/Cas9 technology. The mutation renders the encoded peptide incapable of interacting with integrins. |
