| Primary Identifier | MGI:6781656 | Allele Type | Endonuclease-mediated |
| Gene | Casp8 | Strain of Origin | C57BL/6J |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Aspartic acid codon 387 (GAT) was targeted for change to an alanine codon (GCG)(p.D387A) with an sgRNA (targeting TAATACGACTCACTATAGGACAGAACCACACTTTAGAAGGTTTTAGAGCTAGAAATAGCA) and an ssODN template (CCAAGATCTTTTTCATTCAGGCTTGCCAAGGAAGTAACTTCCAGAAAGGAGTGCCTGATGAGGCAGGCTTCGAGCAACAGAACCACACTTTGGAGGTTGCGAGCTCATCTCACAAGAACTATATTCCGGATGAGGCAGACTTTCTGCTGGGAATGGCTACGGTGAAGAACTGCGTTTCCTACCGAGATCCTGTGAATGGA) using CRISPR/Cas9 technology. The mutation creates an uncleavable form of the encoded peptide. |
