Primary Identifier | MGI:6827133 | Allele Type | Targeted |
Attribute String | Conditional ready | Gene | Sox4 |
Transmission | Germline | Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Using homologous recombination in ES cells, the targeting vector inserted a single loxP into 5'UTR of the Sox4 gene, and a 3' loxP site followed by an frt-flanked neo cassette was inserted downstream of the single coding exon. The Neo cassette was removed by crossing to the Flp recombinase strain and deletion from the 3âUTR was confirmed. |