Primary Identifier | MGI:6827284 | Allele Type | Targeted |
Attribute String | Inserted expressed sequence, Null/knockout | Gene | Pln |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting vector contains from 5' to 3'; a loxP site, an H2B-GFP sequence with 4x polyA sequence, an FRT-flanked neomycin selection cassette, a second loxP site, and followed by a human PLN gene inserted into the start codon of exon 2. The insertion results in a knock-out of the mouse Pln gene. Cre-mediated recombination removes the floxed GFP and neo cassette. |