Primary Identifier | MGI:6860625 | Allele Type | Targeted |
Attribute String | Conditional ready | Gene | Thbs1 |
Transmission | Germline | Strain of Origin | 129S/SvEv |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The allele was generated by inserting 2 loxP sites downstream of exon 3 and exon 7 of the Thbs1 gene, respectively. An FRT (flippase recognition target) site flanked neomycin positive selection cassette was also included right before the 3â²-loxP site. After homologous recombination in SV129-derived ES cells, the neomycin cassette was removed prior to injection of recombinant cells into C57BL/6 blastocysts. |