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Allele : Gt(ROSA)26Sor<em1(CAG-Cd63/EmGFP)Adly> gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Andrew Dudley
Primary Identifier  MGI:6798105 Allele Type  Endonuclease-mediated
Attribute String  Conditional ready, Inserted expressed sequence, Reporter Gene  Gt(ROSA)26Sor
Strain of Origin  C57BL/6J Is Recombinase  false
Is Wild Type  false
molecularNote  Plasmids encoding a guide RNA (GGAGTTGCAGATCACGA) are designed to insert an FRT site, a neo cassette, and a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), followed by a second FRT site, into intron 1 of the Gt(ROSA)26Sor locus. A floxed STOP cassette followed by a mouse CD63 antigen (Cd63) sequence fused to an emerald green fluorescent protein (emGFP) was inserted downstream of the FRT flanked CAG.
  • mutations:
  • Insertion
  • synonyms:
  • CD63-emGFP<l/s/l>,
  • CD63-emGFP<loxP/stop/loxP>,
  • CD63-emGFP<loxP/stop/loxP>,
  • CD63-emGFP<l/s/l>
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1 Feature

Genome

1 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

1 Carried By

0 Driven By

3 Publication categories





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