| Primary Identifier | MGI:6782844 | Allele Type | Targeted |
| Attribute String | Null/knockout, Recombinase, Reporter | Gene | Ankfn1 |
| Transmission | Germline | Strain of Origin | 129S/SvEv x C57BL/6 |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | Using homologous recombination in ES cells, the knock-in vector targeted exon 5 of the Ankfn1 locus and was integrated 21 bp into exon 5, replacing the remainder of the exon with a tdTomato-P2A-split Cre-Neo-WPREBGHpA cassette, which causes frameshifted nonsense mutations downstream, resulting in the loss-of-function allele. Neomycin was excised via FLP mice. |
