| First Author | Modzelewski AJ | Year | 2021 |
| Journal | Cell | Volume | 184 |
| Issue | 22 | Pages | 5541-5558.e22 |
| PubMed ID | 34644528 | Mgi Jnum | J:312811 |
| Mgi Id | MGI:6790349 | Doi | 10.1016/j.cell.2021.09.021 |
| Citation | Modzelewski AJ, et al. (2021) A mouse-specific retrotransposon drives a conserved Cdk2ap1 isoform essential for development. Cell 184(22):5541-5558.e22 |
| abstractText | Retrotransposons mediate gene regulation in important developmental and pathological processes. Here, we characterized the transient retrotransposon induction during preimplantation development of eight mammals. Induced retrotransposons exhibit similar preimplantation profiles across species, conferring gene regulatory activities, particularly through long terminal repeat (LTR) retrotransposon promoters. A mouse-specific MT2B2 retrotransposon promoter generates an N-terminally truncated Cdk2ap1(DeltaN) that peaks in preimplantation embryos and promotes proliferation. In contrast, the canonical Cdk2ap1 peaks in mid-gestation and represses cell proliferation. This MT2B2 promoter, whose deletion abolishes Cdk2ap1(DeltaN) production, reduces cell proliferation and impairs embryo implantation, is developmentally essential. Intriguingly, Cdk2ap1(DeltaN) is evolutionarily conserved in sequence and function yet is driven by different promoters across mammals. The distinct preimplantation Cdk2ap1(DeltaN) expression in each mammalian species correlates with the duration of its preimplantation development. Hence, species-specific transposon promoters can yield evolutionarily conserved, alternative protein isoforms, bestowing them with new functions and species-specific expression to govern essential biological divergence. |
