| Primary Identifier | MGI:6761559 | Allele Type | Targeted |
| Attribute String | Conditional ready, Epitope tag, Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | This allele has three membrane-targeted fluorescent protein sequences - each uniquely flanked with pairs of incompatible lox sites (in the same direction) inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus with a CAG hybrid promoter/enhancer. This allele has three membrane-targeted fluorescent protein sequences - each uniquely flanked with pairs of incompatible lox sites (in the same direction) inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus with a CAG hybrid promoter/enhancer. The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a loxN site, a lox2272 site, a loxP site, a neomycin resistance gene cassette, a second loxN site, the HA-H2B-Cerulean gene (monomeric enhanced cyan fluorescent protein variant mCerulean with N-terminal hemagglutinin tag and 125 amino acid human histone 2B chromatin/nucleus tag sequence), bovine GH polyA signal sequence, a second lox2272 site, the H2B-EGFP-V5 gene (enhanced green fluorescent protein with 127 amino acid human histone 2B chromatin/nucleus tag plus C-terminal paramyxovirus V5 tag sequence), bovine GH polyA signal sequence, a second loxP site, the the H2B-Kate2-His gene (monomeric far-red fluorescent protein mKate2 with N-terminal 125 amino acid human histone 2B chromatin/nucleus tag plus C-terminal 6xHis affinity tag sequence), and bovine GH polyA signal sequence. |
