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Allele : Igs7<tm1(tetO-mCardinal,-L_RABVS)Wick> intergenic site 7; targeted mutation 1, Ian Wickersham
Primary Identifier  MGI:6832819 Allele Type  Targeted
Attribute String  Inducible, Reporter Gene  Igs7
Transmission  Germline Strain of Origin  (129S6/SvEvTac x C57BL/6NCrl)F1
Induced With  doxycycline/tetracycline Is Recombinase  false
Is Wild Type  false
molecularNote  The targeting construct contains a tetracycline-responsive element (TRE; tetO) directing expression of an mCardinal (a basic far red fluorescent protein) and glycoprotein-deleted SAD-B19 rabies virus large structural protein RNA-directed RNA polymerase GTP phosphohydrolase (L_RABVS) variant B19L, separated by a picornavirus 2A element (P2A). The plasmid was then used for FLP-mediated targeted insertion into the Igs7 locus in Ai99 Flp-in embryonic stem (ES) cells derived from (129S6/SvEvTac x C57BL/6)F1 background G4 ES cells.
  • mutations:
  • Insertion
  • synonyms:
  • TRE-tight-mCardinal-P2A-B19L,
  • TRE-CB,
  • TRE-CB,
  • TRE-tight-mCardinal-P2A-B19L
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1 Feature

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

1 Carried By

0 Driven By

3 Publication categories





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