| Primary Identifier | MGI:6781728 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Prtg |
| Transmission | Germline | Strain of Origin | 129/Sv |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | An IRES-lacZ gene replaced exons 3-7 via homologous recombination. An inserted neomycin cassette following the IRES-lacZ gene was removed. The insertion of the IRES-lacZ creates a premature termination and results in a peptide containing only the first 137 amino acids. Western blot analysis confirmed that no full-length protein is expressed in homozygous mice and immunofluorescence staining further confirmed absence of protein in embryos. |
