| First Author | Niino YS | Year | 2021 |
| Journal | PLoS One | Volume | 16 |
| Issue | 7 | Pages | e0253912 |
| PubMed ID | 34197550 | Mgi Jnum | J:307392 |
| Mgi Id | MGI:6720783 | Doi | 10.1371/journal.pone.0253912 |
| Citation | Niino YS, et al. (2021) PKCdelta deficiency inhibits fetal development and is associated with heart elastic fiber hyperplasia and lung inflammation in adult PKCdelta knockout mice. PLoS One 16(7):e0253912 |
| abstractText | Protein kinase C-delta (PKCdelta) has a caspase-3 recognition sequence in its structure, suggesting its involvement in apoptosis. In addition, PKCdelta was recently reported to function as an anti-cancer factor. The generation of a PKCdelta knockout mouse model indicated that PKCdelta plays a role in B cell homeostasis. However, the Pkcrd gene, which is regulated through complex transcription, produces multiple proteins via alternative splicing. Since gene mutations can result in the loss of function of molecular species required for each tissue, in the present study, conditional PKCdelta knockout mice lacking PKCdeltaI, II, IV, V, VI, and VII were generated to enable tissue-specific deletion of PKCdelta using a suitable Cre mouse. We generated PKCdelta-null mice that lacked whole-body expression of PKCdelta. PKCdelta+/- parental mice gave birth to only 3.4% PKCdelta-/- offsprings that deviated significantly from the expected Mendelian ratio (chi2(2) = 101.7, P < 0.001). Examination of mice on embryonic day 11.5 (E11.5) showed the proportion of PKCdelta-/- mice implanted in the uterus in accordance with Mendelian rules; however, approximately 70% of the fetuses did not survive at E11.5. PKCdelta-/- mice that survived until adulthood showed enlarged spleens, with some having cardiac and pulmonary abnormalities. Our findings suggest that the lack of PKCdelta may have harmful effects on fetal development, and heart and lung functions after birth. Furthermore, our study provides a reference for future studies on PKCdelta deficient mice that would elucidate the effects of the multiple protein variants in mice and decipher the roles of PKCdelta in various diseases. |
