| Primary Identifier | MGI:6865661 | Allele Type | Endonuclease-mediated |
| Attribute String | Constitutively active | Gene | Prkcg |
| Strain of Origin | FVB/N | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 technology generated a C to A change at position 27 (c.71C>A) resulting in an alanine to glutamate substitution at amino acid 24 (p.A24E). In addition, a G to A change at position 78 (c.78G>A) that does not change the amino acid (p.R26R) was introduced to prevent the donor DNA from being a suitable target for cas9 cleavage. The A24E mutation keeps PKC gamma in a constitutive active open conformation. mRNA expression is comparable to wild-type but protein is strongly reduced in homozygotes. Cycloheximide trace assay shows increased protein degradation in these mice. Heterozygotes and homozygotes show increased PKC kinase activity despite decreased protein levels. |
