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Allele : Gt(ROSA)26Sor<em27(KRT18-ACE2)Mvw> gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 27, Micheal V Wiles
Primary Identifier  MGI:6877061 Allele Type  Endonuclease-mediated
Attribute String  Epitope tag, Humanized sequence, Inserted expressed sequence Gene  Gt(ROSA)26Sor
Strain of Origin  NOD.Cg-Prkdc<scid> Il2rg<tm1Wjl>/SzJ Is Recombinase  false
Is Wild Type  false
molecularNote  CRISPR/cas9 genome editing and RMCE (recombinase-mediated cassette exchange) are utilized to insert a human keratin 18 promoter directing expression of the full length human ACE2with a C-terminal FLAG epitope tag into a modified Gt(ROSA)26Sor (NSG.RosaBxb-GT/GA) locus. The NOD.Cg-Prkdc Il2rg/SzJ (NSG)-derived zygotes carrying the Gt(ROSA)26Sor (RosaBxb-GT/GA) allele were previously targeted to insert a dual Bxb1 attP-site "safe harbor landing pad" in the first intron of Gt(ROSA)26Sor. The landing pad contains a 48 bp Bxb1 attP-GT sequence and a 48 bp Bxb1 attP-GA sequence separated by a 240 bp spacer of native Gt(ROSA)26Sor sequence. The DNA donor plasmid, carrying two heterologous attB sites and donor transgene, was then microinjected with Bxb1 integrase into the RMCE-ready zygotes (attP-GT/GA).
  • mutations:
  • Insertion
  • synonyms:
  • RMCE[K18-HuACE2],
  • RMCE[K18-HuACE2]
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1 Feature

Genome

1 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

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