| Primary Identifier | MGI:7276204 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence | Gene | Mlh1 |
| Strain of Origin | FVB/NJ x B6(Cg)-Tyr<c-2J>/J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Using an sgRNA (targeting AGGACGACGGCCCGAAGGAA) and an ssODN template (AAGTGGCTGGACAGAGGACGACGGCCCGAAAGAGGGCCTTGCAGAGTACATTGTTGAGTTTCTGAAGAAGGAAGCGGAGATGCTTGCAGACTATTTCTCTGTGGAGATCGATGAGGCGAG) with CRISPR/Cas9 technology, lysine codon 622 (AAA) was changed to glutamic acid (GAA) (c.1864A>G, p.K622E). This is the equivalent of the human p.K618E mutation. |
