| Primary Identifier | MGI:7276208 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence | Gene | Mlh1 |
| Strain of Origin | FVB/NJ x B6(Cg)-Tyr<c-2J>/J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Using an sgRNA (targeting AGGACGACGGCCCGAAGGAA) and an ssODN template (AAGTGGCTGGACAGAGGACGACGGCCCGAAAGAGGGCCTTGCAGAGTACATTGTTGAGTTTCTGAAGAAGGCAGCGGAGATGCTTGCAGACTATTTCTCTGTGGAGATCGATGAGGCGAG) with CRISPR/Cas9 technology, lysine codon 622 (AAA) was changed to alanine (GCA) (c.1864_1865delAAinsGC, p.K622A). This is the equivalent of the human p.K618A mutation. |
