| Primary Identifier | MGI:7261377 | Allele Type | Endonuclease-mediated |
| Attribute String | Humanized sequence, Inserted expressed sequence, Null/knockout | Gene | Tmprss2 |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Cas9 and gRNA Tmprss2-crRNA_5 (protospacer sequence 5â-GTGCTCAGACTGATACCCG-3â) are used to introduce the following sequences at codon 19 in exon 3: GGSG linker, P2A, human TMPRSS2 cDNA coding sequence with SV40 intron inserted for enhanced expression, 1837 bp human TMPRSS2 3âUTR, 112 bp human intergenic sequence immediately 3â of TMPRSS2 polyadenylation region , a cassette comprised of a mouse U6 promoter-driven Tmprss2-gRNA5 guide RNA with 2xPolIII terminator, all in reverse orientation relative to Tmprss2 gene, splice acceptor and SV40 polyadenylation sequence followed by a Flp Recombinase Target (FRT) site and 3-frame ATG short open reading frames. This cassette is designed to trap and truncate any mouse Tmprss2 isoforms that may splice past the hTMPRSS2 cassette. The FRT site allows Flp-mediated collapse of targeted tandem integration events if required. |
