Primary Identifier | MGI:7257816 | Allele Type | Targeted |
Attribute String | Recombinase | Gene | Opn4 |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | true | Is Wild Type | false |
description | The version of this allele that does not contain the neomycin selection cassette, Opn4tm1.1(cre)Saha, has been analyzed. |
molecularNote | Cre recombinase preceded by a human beta-globin intron and nuclear localization signal was inserted immediately after the start codon of the Opn4 gene, replacing the open reading frame. An FRT-flanked pgk-neo cassette followed the cre coding sequence. Homologous recombination in ES cells resulted in replacement of the coding region of Opn4 with this construct. |