| Primary Identifier | MGI:7257820 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Parg |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-CSD |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 31929646 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 31932826. FLP excised by microinjecting 100 ng/ul of FLP mRNA (Miltenyi BIotec Inc. Cat# 130-106-769) into the cytoplasm of IVF-derived zygotes. This resulted in the removal of the neo selection cassette and lacZ reporter leaving behind the floxed critical exon(s). |
