| Primary Identifier | MGI:7293333 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Taok1 |
| Strain of Origin | C57BL/6NCrl | Is Recombinase | false |
| Is Wild Type | false | Project Collection | IMPC |
| molecularNote | This allele was generated at The Centre for Phenogenomics by electroporating Cas9 ribonucleoprotein complexes with single guide RNAs having spacer sequences of GAAAAGCAAATACCGCCGGG targeting the 5' side and AAACATTAAACTAGGCGGTC targeting the 3' side of a critical region (ENSMUSE00000520605) along with a single-strand oligonucleotide encoding a Bxb1 attB site. This resulted in a 1292-bp deletion of Chr11 from 77,465,754 to 77,467,045 (GRCm39) with an insertion of a Bxb1 attB sequence (GGCTTGTCGACGACGGCGGTCTCCGTCGTCAGGATCATACACCGG). |
