| Primary Identifier | MGI:7292552 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Spem2 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6JSmoc |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The CRISPR/Cas9 genome editing system was used to target the entire Spem2 gene, including the 32-bp-long 5â²-untranscribed region, exons 1-3 (except for the last 42 bp of exon 3), and 2 introns in between. A 1894-bp deletion was confirmed by direct sequencing analysis. RT-PCR analysis demonstrated the absence of expression in the testes of homozygous mutant males. |
