| Primary Identifier | MGI:7311604 | Allele Type | Endonuclease-mediated |
| Attribute String | Recombinase | Gene | Prl8a2 |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | Using CRISPR/Cas9 endonuclease-mediated genome editing, a donor plasmid designed to insert a codon improved cre recombinase (iCre) and a polyadenylation sequence immediately downstream of the start codon of the prolactin family 8, subfamily a, member 2 (Prl8a2) gene, a single guide RNA (ACTAGCAGTCTTATCCAACCNGG), and the cas9 nuclease with a puromycin resistance sequence were transfected into G4 embryonic stem (ES) cells. |
