| Primary Identifier | MGI:7335945 | Allele Type | Targeted |
| Attribute String | Conditional ready | Gene | Procr |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | EUCOMM |
| description | Cell line EPD0480_1_A01 was used to create a conditional ready allele which demonstrated germline transmission. |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 155594778 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 155597639. The critical exon(s) is/are thus flanked by loxP sites. Flp-mediated recombination removed the lacZ and neomycin resistance gene generating a conditional ready, floxed allele. |
