Primary Identifier | MGI:7341392 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Trp53 |
Transmission | Germline | Strain of Origin | 129S7/SvEvBrd-Hprt1<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An arginine to histidine substitution (G-to-A at position 515) was introduced at codon 172 (p.R172H) in exon 5. In addition, a loxP site, Trp53 exons 5-11 with a native polyadenylation signal sequence, and FRT site flanked PGK puromycin resistance gene cassette and second loxP site were inserted in intron 4. |