| First Author | Sasaki H | Year | 2021 |
| Journal | Biochem Biophys Res Commun | Volume | 551 |
| Pages | 127-132 | PubMed ID | 33725574 |
| Mgi Jnum | J:305529 | Mgi Id | MGI:6706004 |
| Doi | 10.1016/j.bbrc.2021.03.025 | Citation | Sasaki H, et al. (2021) New inducible mast cell-deficient mouse model (Mcpt5/Cma1(DTR)). Biochem Biophys Res Commun 551:127-132 |
| abstractText | Mast cell-deficient mice are helpful for understanding the roles of mast cells in vivo. To date, a dozen mouse models for mast cell deficiency have been reported. However, mice with a specific depletion of all populations of mast cells have not been reported. We generated knock-in mice, termed Mcpt5/Cma1(DTR) mice, expressing human diphtheria toxin A (DT) receptor under the endogenous promoter of Mcpt5 (also known as Cma1), which encodes mouse mast cell protease-5. Flow cytometry and histological analysis showed that intraperitoneal injection of DT induced almost complete depletion of mast cells in heterozygote Mcpt5/Cma1(DTR/+) mice. The deletion rates of mast cells in peritoneal cavity, mesentery, abdominal skin, ear skin, and glandular stomach were 99.9%, 100%, 98.7%, 97.7%, and 100%, respectively. Passive cutaneous anaphylaxis reaction also revealed mast cell deficiency in ear skin after DT treatment. Other than mast cells, a small percentage of marginal zone B cells in Mcpt5/Cma1(DTR/+) mice were killed by DT treatment. In conclusion, the Mcpt5/Cma1(DTR/+) mouse model is valuable for achieving conditional depletion of all populations of mast cells without inducing a marked reduction in other cells. |
