| First Author | Kitajima Y | Year | 2018 |
| Journal | Skelet Muscle | Volume | 8 |
| Issue | 1 | Pages | 26 |
| PubMed ID | 30139390 | Mgi Jnum | J:328577 |
| Mgi Id | MGI:7336832 | Doi | 10.1186/s13395-018-0174-x |
| Citation | Kitajima Y, et al. (2018) Visualization of PAX7 protein dynamics in muscle satellite cells in a YFP knock-in-mouse line. Skelet Muscle 8(1):26 |
| abstractText | Background: Satellite cells are residential muscle stem cells that express a paired box protein, PAX7. Results: Here, we report a knock-in mouse line expressing a PAX7-enhanced yellow fluorescent protein (YFP) fusion protein that enables visualization of PAX7 protein dynamics in living satellite cells through YFP fluorescence. The YFP fluorescence signals in Pax7-YFP knock-in mice clearly recapitulated the endogenous expression of PAX7 protein in satellite cells. YFP+ satellite cells were efficiently isolated from muscle tissues by fluorescence-activated cell sorting. Homozygous Pax7-YFP knock-in mice (Pax7YFP/YFP) were viable, grew and regenerated muscle normally, and Pax7YFP/YFP mouse-derived satellite cells proliferated, differentiated, and self-renewed as efficiently as those from wild-type (Pax7+/+) mice. Conclusions: Taken together, our Pax7-YFP mouse line is a useful tool to aid the development of stem-cell-based therapies for muscle diseases. Keywords: Knock-in mouse; Muscle regeneration; Myogenesis; Pax7; YFP. |
