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Allele : Gt(ROSA)26Sor<tm8.3(CAG-tdiRFP*)Imayo> gene trap ROSA 26, Philippe Soriano; targeted mutation 8.3, Itaru Imayoshi
Primary Identifier  MGI:7385051 Allele Type  Targeted
Attribute String  Reporter Gene  Gt(ROSA)26Sor
Transmission  Germline Strain of Origin  C57BL/6NSlc
Is Recombinase  false Is Wild Type  false
molecularNote  The targeting construct introduced the tdiRFP713NLS reporter gene expressed under CAG promoter and WPRE sequence into 3’ UTR region. Both cre- and flp-mediated recombination is required to achieve expression of the reporter. FRT-Stop-FRT cassette was removed by crossing pCAG-FLPe strain. Then, loxP-Stop-loxP cassette was removed by injecting pIC-Cre expression plasmid to the fertilized eggs. Therefore, tdiFP713NLS is expressed by all cells throughout the body. The HA-epitope tga was is attched to tdiFP713NLS.
  • mutations:
  • Insertion
  • synonyms:
  • Gt(ROSA)26Sor<tm1.3(CAG-tdiRFP713NLS)Imayo>,
  • Gt(ROSA)26Sor<tm1.3(CAG-tdiRFP713NLS)Imayo>
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1 Feature

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

1 Carried By

0 Driven By

3 Publication categories





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