| Primary Identifier | MGI:7384598 | Allele Type | Targeted |
| Attribute String | Conditional ready, Inserted expressed sequence, Reporter | Gene | Igs2 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | This allele was generated using PhiC31 integrase-mediated transgenesis. A plasmid containing from 5' to 3' a CAG promoter, a loxP-flanked stop sequence, mouse AU040320 (Aavr) fused to mCherry, an F2A self-cleaving peptide sequence, and a mammalian codon-optimized cas9 gene (derived from Streptococcus pyogenes CRISPR associated protein 9 [spCas9]) and PhiC31 integrase RNA was injected into the cytoplasm of C57BL/6 embryos carrying three tandem attP sites in the "safe harbor" Igs2 (H11) intergenic site, Igs2 |
