| Primary Identifier | MGI:7425601 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Col28a1 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NCrl |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Centre for Phenogenomics by electroporating Cas9 ribonucleoprotein complexes with single guide RNAs having spacer sequences of TACTAGGAAAAGGCGTCAAA targeting the 5' side and CGACCACAGACTGATAACCG targeting the 3' side of a critical region (ENSMUSE00000656570) along with a single-strand oligonucleotide encoding a Bxb1 attB site. This resulted in a 952-bp deletion of Chr6 from 8,174,860 to 8,175,811 (GRCm39) with an insertion of a Bxb1 attB sequence (GGCTTGTCGACGACGGCGGTCTCCGTCGTCAGGATCATACACCTT). |
