| Primary Identifier | MGI:7425227 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Adap1 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 3 (functional domain) was replaced by sequences for LacZ, followed by a translational STOP, and a Neo selection cassette flanked by frt sites. Flp-mediated recombination removed the Neo cassette. The modified locus leads to a fusion protein consisting of exons 1 and 2 and LacZ, while the functional protein is missing. Immunoblot analysis confirmed the complete lack of protein expression in the hippocampus of homozygous mutant mice. |
