| Primary Identifier | MGI:7435277 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Lztfl1 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The L1L2_Pgk_P cassette L3L4_pD223_DTA_spec backbone from project CSD50165 was used to generate this allele. The cassette is composed of an FRT site followed by a lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, sV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4. The critical exon 4 is thus flanked by loxP sites. Flp-mediated recombination removed the lacZ and neomycin resistance gene. Cre-mediated recombination deleted exon 4. Western blot analysis confirmed absence of protein in brain tissue lysates. |
