| First Author | Loots GG | Year | 2000 |
| Journal | Science | Volume | 288 |
| Issue | 5463 | Pages | 136-40 |
| PubMed ID | 10753117 | Mgi Jnum | J:61463 |
| Mgi Id | MGI:1355014 | Doi | 10.1126/science.288.5463.136 |
| Citation | Loots GG, et al. (2000) Identification of a coordinate regulator of interleukins 4, 13, and 5 by cross-species sequence comparisons [see comments]. Science 288(5463):136-40 |
| abstractText | Long-range regulatory elements are difficult to discover experimentally; however, they tend to be conserved among mammals, suggesting that cross-species sequence comparisons should identify them. To search for regulatory sequences, we examined about 1 megabase of orthologous human and mouse sequences for conserved noncoding elements with greater than or equal to 70% identity over at least 100 base pairs. Ninety noncoding sequences meeting these criteria were discovered, and the analysis of 15 of these elements found that about 70% were conserved across mammals. Characterization of the largest element in yeast artificial chromosome transgenic mice revealed it to be a coordinate regulator of three genes, interleukin-4, interleukin-13, and interleukin-5, spread over 120 kilobases. |
