| Primary Identifier | MGI:7437558 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Tfpi |
| Transmission | Germline | Strain of Origin | 129S6/SvEv |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cells: SCR012 (129S6/SvEv) |
| molecularNote | A reversed âflipfloxâ vector, consisting of a gene inactivation cassette (an internal ribosome entry site (IRES)-GFP reporter in reverse orientation) flanked by mutant loxP sites and an FRT-flanked PGK-neomycin selection cassette, was inserted into intron 3. The neomycin selection cassette was removed via flp-mediated recombination. The gene-inactivation cassette is transcriptionally silent and does not affect TFPI expression. Cre-mediated recombination is necessary to permanently invert the gene inactivation cassette, express EGFP and block transcription of Tfpi. |
