| First Author | Sugita K | Year | 2015 |
| Journal | Exp Dermatol | Volume | 24 |
| Issue | 7 | Pages | 554-6 |
| PubMed ID | 25846704 | Mgi Jnum | J:325570 |
| Mgi Id | MGI:7285014 | Doi | 10.1111/exd.12711 |
| Citation | Sugita K, et al. (2015) Generation of Helios reporter mice and an evaluation of the suppressive capacity of Helios(+) regulatory T cells in vitro. Exp Dermatol 24(7):554-6 |
| abstractText | Helios is a member of the Ikaros transcription factor family and has been reported to be a marker of thymus-derived regulatory T cells (Treg). Helios is an intracellular protein, however, and hence cannot be used as a marker to separate living Tregs. To solve this problem, we generated Helios reporter mice in which Helios+ cells selectively express Venus, a variant of green fluorescent protein. Most of the Tregs in the thymus expressed Helios, whereas its expression was varied in peripheral lymphoid organs. The Helios+ Treg-population was superior in ability to suppress both antigen-specific and TCR-stimulated T cell responses. We also showed that Helios+ Tregs inhibited the cytokine production by T cells more efficiently than Helios- Tregs. We conclude that Helios reporter mouse strain is a useful tool to study function of Helios and that Helios+ Tregs represent the highly suppressive population. |
