| Primary Identifier | MGI:7444368 | Allele Type | Targeted |
| Attribute String | Dominant negative, Epitope tag, Inducible, Recombinase | Gene | Atoh1 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| molecularNote | The targeting construct was designed to introduce a loxP site into 5'UTR of Atoh1, followed by the hyperactive phosphomutant (9 amino acids S/T to A changes) Atoh1 sequence Atoh1(9S/T-A) merged to the P2A-CreERT2-hCD2 then to a neomycin cassette inserted such that the 3'UTR was not disrupted. Following successful germline transmission, the mice heterozygous for the targeting construct were crossed onto PGK-Cre line in order to remove both the neo-selection cassette and the endogenous Atoh1 locus at the same time. |
