| Primary Identifier | MGI:7446375 | Allele Type | Targeted |
| Attribute String | Conditional ready, Inserted expressed sequence | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting vector is constructed based on the Ai6 vector from Addgene with (from 5' to 3') a CAG (CMV enhancer/chicken beta-actin) promoter, an FRT site, a floxed STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), and murine B cell leukemia/lymphoma 2 (Bcl2) cDNA followed by a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), a polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette. The targeting vector is inserted into the Gt(ROSA)26Sor locus. |
