| Primary Identifier | MGI:7447104 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Nxpe4 |
| Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | IMPC |
| molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TTCTTGGTCCATAGTAGGCA and GTGACCAAGTCCAACAGAAA, which resulted in a 464 bp deletion beginning at Chromosome 9 position 48,393,961 bp and ending after 48,394,424 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000334056 (exon 7) and 402 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 275 and early truncation 19 amino acids later. |
