| Primary Identifier | MGI:7448467 | Allele Type | Endonuclease-mediated |
| Attribute String | Modified regulatory region | Gene | Rr253 |
| Strain of Origin | C57BL/6J | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Using zygotes that already carry the Rr253em4Kmm NFIL3âC/EBP binding site 1&2-deletion allele, binding site 3 in the Zeb2 enhancer, located ~165 kb upstream, were targeted with an sgRNA (targeting CCACCAGCCGGTGACACACA) and an ssODN template (GCTGAAGTTCACCTCCCCATGGGGGAATGAATCATCAAAATAACCGGTGGAAAACAAGCTAGATCTGACTTGAGCAGTGTGTGTCACCGGCTGGTGGAATTTTTAGAAAGGCAGCAGTTTGGGCTCATCACTGCGGTT) using CRISPR/Cas9 technology, resulting in an allele lacking binding sites 1, 2 and 3. |
