| Primary Identifier | MGI:7448510 | Allele Type | Targeted |
| Attribute String | Epitope tag, Recombinase, Reporter | Gene | Agrp |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | true | Is Wild Type | false |
| molecularNote | The targeting construct introduced a cassette containing the following features, without the nuclear localization signal, using a nonoptimal initiation codon, a cre recombinase gene fused with EGFP (cre/EGFP) with N-term sequence removed, and a myc tag with an additional 3â² untranslated region from the Myc gene that promotes a short messenger RNA half-life. Flp-mediated recombination removed the FRT-flanked neomycin resistance cassette introduced downstream of the fusion gene. It is a revised "version 2" of the allele Agrptm1.1(cre/EGFP)Stei designed to have an attenuated expression of Cre:GFP. |
