| Primary Identifier | MGI:7465046 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Mcph1 |
| Transmission | Germline | Strain of Origin | C57BL/6N |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | LoxP site was inserted into intron 7 together with a self-excision neomycin cassette flanked by Rox sites and a second loxP site was inserted into intron 8. The neomycin cassette was self-excised. Exon 8 was deleted via cre-mediated recombination in the germline. RT-PCR confirmed the in-frame deletion of the central domain (exon 8). Western blot analysis using an antibody against the middle domain confirmed its absence. |
